Laboratory protocols

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Protocol overviews
Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation
Fungal Molecular Biology
Determination of Plasmid Copy Number in Yeast
Lambda IIBargem7B
RNA extraction: miniprep
A lambda/plasmid Cre/lox hybrid vector for large genomic (18kb) fragment insertions and fungal genomic library construction
RNA extraction: large scale prep
Using Non-homologous End-Joining-Deficient Strains for Functional Gene Analyses in Filamentous Fungi
PCR from spores
RNA extraction: Isolation of mRNA
Protein methods
Enzyme Immobilization Using Chitosan-Xanthan Complexes (including Aspergillus sojae protease type XIX)
Cell Wall Fractionation for Yeast and Fungal Proteomics
Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers (xylanase from Aspergillus niger)
A Multi-Step Chromatographic Strategy to Purify Three Fungal Endo-β-Glucanases
PCR
Multiplex Detection of Aspergillus Species (real-time PCR)
Differentiation of Fungi Using Hybridization Probes on the LightCycler®
Model systems: in vivo
Animal Models to Evaluate Anti-infective Pharmacodynamics
Assaying mycotoxins
Chromatographic Method for AlternariaToxins in Apple Juice
HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes
Analysis of Multiple Mycotoxins in Food
Detection in clinical samples
Fungal Diagnostics: Review of Commercially Available Methods
Protocol for homogenisation of CF sputum samples
whole-cell screening
A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus
Methods for DNA sequencing
Library Preparation and Data Analysis Packages for Rapid Genome Sequencing
Aflatoxins
Measurement of Aflatoxins Using Capillary Electrophoresis
Additional methods & reference
Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid-Phase Microextraction-Gas Chromatography/Mass Spectrometry

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